目的 研究海南产和广东产沉香木中真菌的组成及差异。方法 利用Illumina HiSeq测序平台对沉香木中真菌基因组测序并进行生物信息学分析。 结果 海南省内各地区沉香木中真菌的菌落结构接近,广东省内沉香木的真菌菌落结构接近。广东产和海南产沉香木中真菌组成存在明显差异,海南产沉香木真菌多样性和丰富度高于广东产沉香木的真菌。子囊菌亚门Ascomycota为沉香木的优势门,担子菌亚门Basidiomycota次之。子囊菌亚门是海口、万宁、乐东、儋州和东莞沉香木真菌的优势种群,担子菌亚门是化州沉香木真菌的优势种群。伞菌纲Agaricomycetes为广州化州沉香木的优势种群。粪壳菌纲Sordariomycetes为东莞和儋州沉香木的优势种群。座囊菌纲Dothideomycetes为海口、乐东和万宁地区沉香木的优势种群。Pleosporales分别为东莞、海口、万宁和乐东地区沉香木真菌的优势目,Trechisporales为化州沉香木真菌的优势目,Polyporales为儋州沉香木真菌的优势目。Lignosphaeria为海口和万宁沉香木真菌的优势属,Perenniporia 和Pyrigemmula分别为儋州和乐东沉香木真菌的优势属,Phaeoacremonium为化州和东莞沉香木真菌的优势属。不同地区沉香木的优势种群不同。结论 海南产沉香木真菌多样性和丰富度高于广东产沉香木,不同地区沉香木真菌分布有差异。
Abstract
OBJECTIVE To characterize the fungal communities in agarwood wood from Hainan province and Guangdong province. METHODS Fungal ITS genes in the agarwood wood were analyzed by high-throughput pyrosequencing using Illumina HiSeq system and bioinformatically. RESULTS The fungal microbiomes exhibited much similarity among the samples from different regions in each province. And there were notable differences between Guangdong province and Hainan province. Moreover, the fungal communities in the agarwood wood from Hainan Province showed higher diversity and richness than that in the agarwood wood from Guangdong province. At the phylum level, Ascomycota most dominated in the agarwood wood, followed by Basidiomycota. Ascomycota dominated in the agarwood wood from Haikou, Wanning, Ledong, Danzhou and Dongguan regions. Basidiomycota dominated in the agarwood wood from Huazhou region. At the class level, agaricomycetes dominated in the agarwood wood from Huazhou region. Sordariomycetes dominated in the agarwood wood from Dondguan and Danzhou regions, Dothideomycetes dominated in the agarwood wood from Haikou, Ledong and Wanning regions. At the order level, Pleosporales dominated in the agarwood wood from Haikou, Ledong, Wanning and Dondguan regions. Trechisporales and Polyporales dominated in the agarwood wood from Huazhou and Danzhou regions, respectively. At the genus level, Lignosphaeria dominated in the agarwood wood from Haikou and Wanning regions, Perenniporia and Pyrigemmula dominated in the agarwood wood from Ledong and Danzhou regions, respectively. Phaeoacremonium dominated in the agarwood wood from Huazhou and Dondguan regions. Agarwood wood from different regions contained different dominate fungal populations. CONCLUSION The fungi in the agarwood wood from Hainan province has higher diversity and richness than that in the agawood wood from Guangdong province. Agarwood wood from different regions has distinct fungal communities.
关键词
白木香 /
沉香木 /
真菌 /
多样性 /
丰富度
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Key words
Aquilaria sinensis (Lour.) Gilg /
agarwood wood /
fungi /
diversity /
richness
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中图分类号：
R282
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参考文献
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基金
国家重点研发计划项目资助(2018YFC1706401);中组部“万人计划”项目资助(99950534);国家中药材产业技术体系资助(CARS-21);国家自然科学基金项目资助(81303312,81173481);中国医学科学院医学与健康科技创新工程资助(2016-I2M-2-003,2017-I2M-1-013)
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